Fibronectin Antibody

貨號 Cat. No.
3630-100
規格 Size
100 µg

Description

pstrongAntibody Target :/strongFibronectin/p
pbHost: /bRabbit/p
pbAntibody Type: /bPolyclonal/p
pbIsotype: /bRabbit IgG/p
pbAntibody Form: /bAffinity Purified Antibody/p
pbImmunogen Sequence: /bPurified fibronectin from human plasma/p
pbAccession#: /bN/A/p
pbGene ID: /bN/A/p
pbAppearance: /bColorless liquid/p
pbConcentration: /b0.5 mg/ml/p
pbFormulation: /b100 micro;g (0.5mg/ml) antigen affinity purified rabbit anti-Fibronectin polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 0.1% BSA and 0.02% thimerosal./p
pbPurification: /bAffinity purified/p
pbSpecies Reactivity: /bhu/p
pbApplication: /bWB/IHC//p
pbApplication amp; Usage: /bWestern blotting (0.5 ndash; 2.0 micro;g/ml), immunohistochemistry (20-40 micro;g/ml). However, the optimal concentrations should be determined individually./p
pbStorage Temp.: /b-20 deg;C/p
pbShipping : /bgel pack/p
pbShelf Life: /b12 months/p
pbHandling: /bThe antibody solution should be gently mixed before use./p
pbBrief Background Descriptions: /bFibronectin is a glycoprotein synthesized in the liver for the circulating blood plasma form, and is synthesized by many mesenchymal cells, for the extracellular matrix form. It is composed of two similar, but not identical protein chains, which are linked by two disulfide linkages at the C-terminal end of the chains. The chains are composed of domains which have specific secondary structures linked together by regions which are especially susceptible to proteolytic action. For this reason, detection by immunoblot (western) may show considerable variability in the observed apparent molecular weights, which is predicated on the source of the fibronectin, and to what degree proteolysis has occurred. Bands approximately 225 kDa should be observed after SDS-PAGE when reducing and denaturing conditions are used/p

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