Ferric Reducing Antioxidant Power (FRAP) Assay Kit (Colorimetric)

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Storage Conditions: 4°C

Shelf life: 12 months

Summary :

• Detection method- Absorbance (594 nm)
• Species reactivity- Foodstuff, biological fluids, drinks
• Applications- Assay measuring either the combination of both small molecule and protein antioxidants or small molecules antioxidants

Detection Method: Absorbance (OD 594 nm)

Features & Benefits:

• Simple procedure; takes less than 2 hours
• Fast and convenient

Description:

Ferric reducing antioxidant power (FRAP) assay is a widely used method that uses antioxidants as reductants in a redox-linked colorimetric reaction, wherein Fe3+ is reduced to Fe2+. Ferric (Fe3+) to ferrous (Fe2+) ion reduction at low pH causes formation of a colored ferrous-probe complex from a colorless ferric-probe complex. Antioxidants are molecules which act as reducing agents by donating electrons to free radicals to stabilize them and minimize the damage caused by free radicals to DNA, cells and organ systems. Antioxidants include substances such as polyphenols; flavonoids; vitamins and enzymes like glutathione peroxidase and superoxide dismutase. They are known to have beneficial health effects such as lowering the risk of cancer, heart disease and neurodegenerative disorders and are abundantly found in plants, fruits, vegetables, beverages and natural products. BioVision’s FRAP assay kit provides a quick, sensitive and easy way for measuring antioxidant capacity of various biological samples. The assay is high-throughput adaptable and can detect antioxidant capacities as low as 0.2 mM Fe2+ equivalents