Proteins and Peptides
FBN1 Antibody (LS-B2355)
- 貨號 Cat. No.
- LS-B2355
- 規格 Size
- 50 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2355 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C23548. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Fibrillin 1 (FBN1) |
| Synonyms: | FBN1, fbn1, fibrillin 1 |
| Host: | FBN1 antibody was produced in Mouse |
| Antigen Species: | FBN1 antibody was raised against Bovine |
| Clonality: | Monoclonal |
| Isotype: | IgG1,k |
| Immunogen: | FBN1 antibody was raised against microfibrils from zonular apparatus of bovine eye. Epitope: aa 451-909 |
| Antibody Specificity: | Recognizes bovine Fibrillin-1 (350kD). Reactivity with deletion mutants indicates that the epitope spans between aa451-909 of fibrillin-1. In Immunohistochemistry staining of human skin, decorates elastic framework. More specifically the oxytalantic network close to the epidermis. Cultures of normal human skin fibroblasts show discrete staining of extracellular fibrils. Species cross-reactivity: human. |
| Antigen Modification: | aa451-909 |
| Predicted Species Reactivity: | Human |
| Purification: | Protein G Column |
| Presentation: | PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. |
| Recommended Storage: | Long term: Add glycerol (40-50%) -20°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2355 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2355 was determined to be 5 ug/ml. Positive control: Normal skin fibroblasts. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Immunohistochemistry - Frozen (IHC-Fr), Western blot (WB) (1 - 2 µg/ml), Immunofluorescence (IF) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µl |
| Concentration: | 0.2 mg/ml |
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