F2RL1 Antibody (LS-B2321)

貨號 Cat. No.: LS-B2321
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2321
Antibody Validation:	This antibody replaces catalog number LS-C38064. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Proteinase-Activated Receptor 2 (F2RL1)
Synonyms:	F2RL1, coagulation factor ii thrombin receptor-like 1, f2rl1, g protein-coupled receptor-11, gpr11, par-2, PAR2, protease-activated receptor 2, proteinase activated receptor 2
Family:	GPCR
Subfamily:	Proteinase-activated
Host:	F2RL1 antibody was produced in Mouse
Antigen Species:	F2RL1 antibody was raised against Human
Clonality:	Monoclonal
Isotype:	IgG2a
Antigen Type:	Synthetic peptide
Immunogen:	F2RL1 antibody was raised against synthetic peptide corresponding to the N-terminal of the human PAR2 receptor.
Antibody Specificity:	Recognizes native peptide and the N-terminus of the human PAR2 receptor. Does not cross-react with PAR1.
Antigen Modification:	N-Terminus
Purification:	Protein A Column
Presentation:	PBS, pH 7.4, 0.1% sodium azide.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2321 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2321 was determined to be 5 ug/ml. Immunofluorescence: 1-3 ug/ml. ELISA: 0.1-1 ug/ml. Western Blot: 1-3 ug/ml.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (1 - 3 µg/ml), Immunofluorescence (IF) (1 - 3 µg/ml), ELISA (0.1 - 1 µg/ml) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	0.5 mg/ml