Proteins and Peptides
F2R Antibody (LS-B2651)
- 貨號 Cat. No.
- LS-B2651
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2651 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C38062. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Thrombin Receptor (F2R) |
| Synonyms: | F2R, cf2r, coagulation factor ii receptor, HTR, PAR-1, par1, protease-activated receptor 1, thrombin receptor, TR |
| Family: | GPCR |
| Subfamily: | Proteinase-activated |
| Host: | F2R antibody was produced in Mouse |
| Antigen Species: | F2R antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Antigen Type: | Synthetic peptide |
| Immunogen: | F2R antibody was raised against a synthetic peptide corresponding to the N-terminal region of the human thrombin receptor PAR1. |
| Antibody Specificity: | Recognizes the cleaved and intact forms of human thrombin receptor PAR1, N-terminus. Does not cross-react with PAR2. |
| Antigen Modification: | N-Terminus |
| Purification: | Protein A Column |
| Presentation: | PBS, pH 7.4, 0.1% sodium azide |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2651 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2651 was determined to be 10 ug/ml. Immunofluorescence (Methanol or acetone fixed CHRF cells): 1-3 ug/ml. Flow Cytometry (Methanol or acetone fixed CHRF cells): 10-20 ug/ml. ELISA: 0.1-1.0 ug/ml. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Flow Cytometry (Flo) (10 - 20 µg/ml), Immunofluorescence (IF) (1 - 3 µg/ml), ELISA (0.1 - 1 µg/ml) (Optimal dilution to be determined by the researcher) |
| Not Recommended Uses: | WB |
| Size: | 50 µg |
| Concentration: | 0.5 mg/ml |
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