Proteins and Peptides
EZH2 Antibody (LS-B2528)
- 貨號 Cat. No.
- LS-B2528
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2528 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C6747. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Enhancer Of Zeste (EZH2) |
| Synonyms: | EZH2, enhancer of zeste, ENX-1, ENX1, EZH1, KMT6, KMT6A, MGC9169 |
| Host: | EZH2 antibody was produced in Rabbit |
| Antigen Species: | EZH2 antibody was raised against Human |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Synthetic peptide |
| Immunogen: | EZH2 antibody was raised against synthetic peptide derived from the internal region of the human EZH2 (ENX1) protein. |
| Antibody Specificity: | Recognizes the human EZH2 protein. On Western blots, it identifies bands at ~100kD and ~125kD, which may represent different isoforms of the protein. Reactivity has been confirmed with human Jurkat T cell leukemia, PC-3 prostate adenocarcinoma, BC-1 B cell lymphoma, Nalm-6 pre-B lymphocyte and HeLa cervical adenocarcinoma cell lysates by Western Blot and with formalin-fixed, paraffin-embedded (FFPE) human breast cancer, lymphoma, and normal prostate, esophagus, pancreas, stomach, liver, and brain tissues by immunohistochemistry. Species sequence homology: Mouse. |
| Antigen Modification: | Internal |
| Purification: | Immunoaffinity Chromatography |
| Presentation: | PBS, pH 7.4, 0.1% sodium azide. |
| Recommended Storage: | Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2528 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2528 was determined to be 5 ug/ml. Western Blot: 0.5-2 ug/ml. Bands at ~100kD and ~125kD. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (0.5 - 2 µg/ml), ELISA (0.1 - 1 µg/ml) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 0.25 mg/ml |
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