Proteins and Peptides
ESR1 Antibody (LS-B1578)
- 貨號 Cat. No.
- LS-B1578
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B1578 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C41415. It has been validated for use in the following assays: IHC. |
| Antibody Target: | Estrogen Receptor Alpha (ER Alpha/NR3A1) (ESR1) |
| Synonyms: | ESR1, er alpha, er-alpha, Eralpha, esr1, Estrogen receptor alpha, nr3a1 |
| Family: | NHR |
| Subfamily: | NR3 Steroid receptor |
| Host: | ESR1 antibody was produced in Mouse |
| Antigen Species: | ESR1 antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Antigen Type: | Recombinant protein |
| Immunogen: | ESR1 antibody was raised against recombinant protein |
| Antibody Specificity: | Ni-NTA purified recombinant human ER-alpha (410-592aa) expressed in E. Coli strain BL21 (DE3) |
| Antigen Modification: | aa410-592 |
| Purification: | Protein A Column |
| Presentation: | PBS, pH 7.4, 0.03% sodium azide. |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B1578 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1578 was determined to be 1:800. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (1:800), Western blot (WB) (1:500 - 1:2000) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 1 mg/ml |
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