ERCC3 / XPB Antibody (LS-B2815)

貨號 Cat. No.: LS-B2815
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2815
Antibody Validation:	This antibody replaces catalog number LS-C6713. It has been validated for use in the following assays: IHC-P.
Antibody Target:	ERCC3 / XPB
Synonyms:	ERCC3 Antibody, BTF2 Antibody, dna-repair protein complementing xp-b cells Antibody, GTF2H Antibody, RAD25 Antibody, TFIIH Antibody, XPB Antibody
Host:	ERCC3 antibody was produced in Mouse
Antigen Species:	ERCC3 antibody was raised against Human
Clonality:	Monoclonal
Isotype:	IgG2b
Antibody Clone Name:	3G4
Antigen Type:	Synthetic peptide
Immunogen:	ERCC3 antibody was raised against synthetic peptide corresponding to aa242-261 of human ERCC-3.
Antibody Specificity:	Recognizes an epitope located between aa242-261 of ERCC-3 (largest subunit of TFIIH).
Antigen Modification:	aa242-261
Purification:	Protein G Column
Presentation:	0.1 M Tris-HCl, pH 7.5.
Recommended Storage:	Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
Antibody Usage Summary:	Immunohistochemistry: LS-B2815 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2815 was determined to be 5-10 ug/ml. Immunoprecipitation: Immunoprecipitates transcriptionally active TFIIH in a reconstituted assay.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 - 10 µg/ml), Western blot (WB), Immunoprecipitation (IP), ELISA (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	0.5 mg/ml