Proteins and Peptides
ERBB2 Antibody (LS-B2133)
- 貨號 Cat. No.
- LS-B2133
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2133 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C23217. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | ERBB-2 Receptor Protein Tyrosine Kinase (ERBB2) |
| Synonyms: | ERBB2, c-neu, erbb2, her-2/neu, Her2, herstatin, neu oncogene, oncogene ngl, p185erbb2, tkr1, tyrosine kinase receptor her2, v-erb-b2 |
| Family: | Protein Kinase |
| Subfamily: | EGF Receptor |
| Host: | ERBB2 antibody was produced in Rabbit |
| Antigen Species: | ERBB2 antibody was raised against Human |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Synthetic peptide |
| Immunogen: | ERBB2 antibody was raised against a KLH-linked peptide (AENPEYLGLDVPV) corresponding to aa1243-1255 from the human c-erbB-2/HER-2/neu oncoprotein. |
| Antibody Specificity: | Recognizes c-erbB-2 (185kD), but not c-erbB-1(EGF-R), c-erbB-3 or c-erbB-4. Cross-reacts with reported with monkey, rat and mouse. |
| Antigen Modification: | aa1243-1255 |
| Predicted Species Reactivity: | Monkey, Mouse, Rat |
| Purification: | Protein A Column |
| Presentation: | 10 mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. |
| Recommended Storage: | Long term: -20°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2133 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2133 was determined to be 5 ug/ml. Western Blot: 0.5-2 ug/ml detects c-erbB-2 in RIPA lysates from A431, MDA, MCF-7, LNCap, and c-erbB-2 transfected 3T3/NIH cells. Immunoprecipitation: 4 ug immunoprecipitated c-erbB-2 from 500 ug of A431, LNCap, and c-erbB- 2 transfected 3T3/NIH RIPA lysate. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Immunohistochemistry - Frozen (IHC-Fr), Western blot (WB) (0.5 - 2 µg/ml), Immunoprecipitation (IP) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
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