Kits
Epidermal-Growth-Factor-Receptor (EGF-R), ELISA
- 貨號 Cat. No.
- K 7110
- 規格 Size
- 96 Tests
- 品牌 Brand
- IDS
Description
| Article number: | K 7110 |
| Method: | ELISA |
| Tests: | 96 Tests |
| Matrix: | Tissue |
| Volume: | 200 µl |
| Incubation time: | 1h; 0.5h; 24h; 2x1h; 15min |
| Standards: | 3-24 fmol/ml |
| Polyclonal growth factors e.g. the epidermal growth factor (EGF) have been recognized as important determinants in the regulation of cellular proliferation and differentiation as shown in cell culture. EGF mediates its effects through receptors. The EGF-receptor (EGF-R) is a glycoprotein of 170.000 Dalton. It consists of an extra cellular domain for EGF binding, a transmembrane domain for signal transduction and an intracellular domain with tyrosine-kinase activity. Binding of EGF leads to a Ca2+ release by activation of Calmodulin-dependent enzymes. Overexpression or amplification of EGF-R in breast cancer is associated with autonomous tumor growth and poor clinical prognosis. The most widely used method of receptor identification has been a radioligand - assay with 125I-labelled EGF. The advantage of the radioligand assay is the measurement of native receptor. On the other hand, this testsystem is combined with a lot of disadvantages (e.g. great amount of tumormaterial, radioactivity, poor reproducibility, time-consuming calculation). In the offered test system EGF-R is determined by specific antibodies. This immunoassay shows good reproducibility, small sample volume, and is easy to calculate. The sensitivity for h EGF-R in ELISA - technique is 0.4 fMol/ml. |