Enteropeptidase/Enterokinase Inhibitor Screening Kit (Fluorometric)

Alternate Name :

Tag Line :

Simple Assay to Screen Enteropeptidase Inhibitors

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection Method – Fluorescence (Ex/Em = 380/500 nm)
• Application:
- Screening potential inhibitors of Enteropeptidase/Enterokinase
- Characterize/study Enteropeptidase/Enterokinase inhibitors

Detection Method:

Features & Benefits:

• Rapid & simple
• Reliable test for screening potential inhibitors of Enteropeptidase/Enterokinase
• Includes Enteropeptidase-specific Inhibitor [Aprotinin]

Description:

Enteropeptidase (Enterokinase, EC 3.4.21.9) is a serine protease involved in activation of trypsinogen to trypsin, which in turn results in the activation of various digestive enzymes. It recognizes a highly specific amino acid sequence ‘DDDDK’ and cleaves after the lysine residue. High specific activity of Enteropeptidase has been utilized in cleaving a variety of native or fusion proteins containing the above recognition. In Biovision’s Enteropeptidase inhibitor screening kit, we have utilized a peptide substrate containing the Enteropeptidase recognition sequence along with a fluorescent label ‘AFC’. Enteropeptidase catalyzes the cleavage of this substrate and releases the AFC molecule, which can be easily quantified by measuring its fluorescence at Ex/Em = 380/500 nm. In the presence of potent Enteropeptidase inhibitor, the hydrolyzation of the substrate will be impeded. The kit provides a rapid, simple & reliable test for screening potential inhibitors of Enteropeptidase.