eNOS Antibody
- 貨號 Cat. No.
- 3426R-100
- 規格 Size
- 100 µg
Description
Antibody Target :eNOS
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Antibody Form: Affinity Purified Antibody
Immunogen Sequence: Synthetic peptide surrounding amino acid 602 of rat eNOS
Accession#: EDL99377
Gene ID: 24600
Appearance: Colorless liquid
Concentration: 0.5 mg/ml
Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Purification: Affinity purified
Species Reactivity: hu/ms/rt
Application: WB
Application & Usage: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes 140 kDa eNOS of human, mouse, and rat origins. Reactivity to other species has not been tested.
Storage Temp.: -20 °C
Shipping : gel pack
Shelf Life: 12 months
Handling: The antibody solution should be gently mixed before use.
Brief Background Descriptions: eNOS (Endothelial nitric-oxide synthase) is an important enzyme in the cardiovascular system. eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495. Several protein kinases including Akt/PKB, PKA and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli. In contrast, bradykinin and hydrogen peroxide activate eNOS activity by promoting Thr495 dephosphorylation.