Enolase Activity Colorimetric/Fluorometric Assay Kit

Alternate Name : 2-phospho-D-glycerate hydrolase Activity Colorimetric/Fluorometric Assay Kit, 2-phosphoglycerate dehydratase Activity Colorimetric/Fluorometric Assay Kit

Tag Line :

Only kit on the market to measure enolase activity

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection method- Absorbance (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm)
• Applications:
- Measurement of enolase activity in various tissues/cells
- Analysis of glycolytic metabolism in different tissues/cells
- Mechanistic studies of various cancers
- Screening anti-cancer drugs

Detection Method: Absorbance (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm)

Features & Benefits:

• Rapid and easy-to-use.
• Highly-sensitive.
• This assay kit can measure less than 0.04 mU of enolase activity in a variety of samples.

Description:

Enolase (EC 4.2.1.11), also called 2-phospho-D-glycerate hydrolase or 2-phosphoglycerate dehydratase, is a key enzyme in glycolysis. It converts 2-phosphoglycerate to phosphoenolpyruvate (PEP) & also catalyzes the reverse reaction, PEP to 2-phosphoglycerate under anabolic conditions during gluconeogenesis. In mammals, Enolase consists of three subunits: α, β and γ that combine into 5 isozymes, 3 of which are commonly found in human tissues. This enzyme exists in all organisms, which can undergo glycolysis. Besides its role in glycolysis, enolase has other functions too. Recent studies have shown that increased activity of enolase is associated with tumor invasion and metastasis; therefore, precise measurement of enolase activity may be of great interest for tumor diagnosis and prognosis. In BioVision’s Enolase Activity Assay Kit, enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate, which is subsequently used to generate an intermediate product. The intermediate product stoichiometrically reacts with OxiRed™ probe to generate color (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm). This simple & sensitive assay Kit can detect enolase activity less than 0.04 mU in a variety of samples.