Enhanced Green Fluorescent Protein (EGFP)

Alternate Names / Synonyms: Recombinant EGFP Protein, Enhanced Green Fluorescent Protein

Gene Symbol: N/A

Accession #: N/A

Gene ID: N/A

Gene Source: N/A

Recombinant Host: E. coli

Appearance: Liquid

Physical Form: Ready to use liquid in PBS with no additives

Molecular Weight: 32.7 kDa

Purity by SDS-PAGE: ≥97%

Purity by HPLC: ≥97%

Endotoxin Level: <0.1 ng/μg

Biological Activity: N/A

Reconstitution: N/A

Storage Temp.: -80°C

Shipping: Gel pack

Background Information: The recombinant EGFP is expressed and purified from transformed E. coli using a method that ensures high purity and maximal GFP fluorescence. Endotoxin has been removed, so the protein is suitable for in vivo injection or cell culture applications. The recombinant EGFP (Gene Bank Accession no. U57607) is a 32.7 kDa monomer with 293 amino acids tagged with 6XHis on both N- and C-terminus. Therefore, the EGFP can be detected by both His-tag antibody (Cat.# 3998-100) and GFP antibody (Cat.# 3999-100) on Western Blot analysis. The excitation and emmission spectra for the recombinant EGFP is identical to GFP purified from Aequorea Victoria (Chalfie et al, 1994), with Ex/Em = 488/507 nm (e489 nm = 55,000 M-1 Cm-1; e280 nm = 21980 M-1 Cm-1).

Handling: Centrifuge the vial prior to opening.