ELISA Kit for IgM Antibody to Hepatitis B Core Antigen

The purified Anti-µ-chain is coated on the solid phase of multi-wells. Serum sample, HBcAg and Horseradish peroxidase labeled with Anti-HBc (conjugated) are added to coated wells. After incubation, if Anti-HBc-IgM is present in the sample, a complex of Anti-µ-chain-Anti-HBc-IgM-HBcAg-Anti-HBc labeled with HRP will form. Wash wells to remove other unbounded serum components, incubate with substrates (TMB) to form a colored product, and measure the absorbance at 450 nm to indicate the presence or absence of Anti-HBc-IgM in the sample. The test is special, sensitive, reproducible and easy to operate.