ELISA Kit for IgM Antibody to Hepatitis A Virus

The purified Anti-µ-chain is coated on the solid phase of multi-wells. Serum sample, HAVAg and Horseradish peroxidase labeled with Anti-HAV (conjugated) are added to coated wells. After incubation, if HAV-IgM is present in the sample, a complex of Anti-µ-chain-HAV-IgM-HAVAg-Anti-HAV labeled with HRP will form. Wash wells to remove other unbounded serum components, incubate with substrate (TMB) to form a colored product, and measure the absorbance at 450 nm to indicate the presence or absence of HAV-IgM in the sample. The test is special, sensitive, reproducible and easy to operate.