ELISA Kit for Antibody to Hepatitis B Core Antigen

The purified recombinant HBcAg is coated on the solid phase of multi-wells. Serum sample and Horseradish peroxidase labeled with Anti-HBc (conjugated) are added to coated wells, and form competitive combination. After incubation, if Anti-HBc content is high in the sample, a complex of HBcAg-Anti-HBc will form, and little complex of HBcAg-Anti-HBc labeled with HRP will form. Wash wells to remove these complex, incubate with substrates (TMB), subsequently there is little color change. If Anti-HBc is not present in the sample, there is much color change. The test is special, sensitive, reproducible and easy to operate.