ELAVL1 Antibody (LS-B2227)

貨號 Cat. No.: LS-B2227
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2227
Antibody Validation:	This antibody replaces catalog number LS-C15836. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Hur RNA Binding Protein (ELAVL1)
Synonyms:	ELAVL1, hur rna binding protein
Host:	ELAVL1 antibody was produced in Rabbit
Antigen Species:	ELAVL1 antibody was raised against Human
Clonality:	Polyclonal
Isotype:	IgG
Antigen Type:	Synthetic peptide
Immunogen:	ELAVL1 antibody was raised against kLH-conjugated, synthetic peptide corresponding to aa1-13 (MSNGYEDHMAEDC) of human HuR. The immunizing sequence is identical in chicken, mouse, and rat.
Antibody Specificity:	Recognizes HuR, Mr ~35kD. Species cross-reactivity: Human and mouse. Predicted to cross-react with rat based on sequence homology.
Antigen Modification:	aa1-13
Predicted Species Reactivity:	Mouse, Rat
Purification:	Affinity Chromatography
Presentation:	PBS, 0.05% sodium azide, before the addition of glycerol to 40%
Recommended Storage:	Long term: -20°C; Short term: +4°C
Antibody Usage Summary:	Immunohistochemistry: LS-B2227 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2227 was determined to be 5 ug/ml. Western Blot: 0.5-2.0 ug/ml. Detected HuR in RIPA lysates from 3T3/A31 cells. Immunoprecipitation: 4 ug immunoprecipitated HuR from 500 ug of 3T3/A31 RIPA lysate.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB), Immunoprecipitation (IP) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	0.5 mg/ml