Elastase, Human Neutrophil

Alternate Names / Synonyms: Leukocyte Elastase, ELA2

Gene Symbol: N/A

Accession #: N/A

Gene ID: N/A

Gene Source: N/A

Recombinant Host: Human Neutrophil

Appearance: Lyophilized protein

Physical Form: Salt- free lyophilized solid

Molecular Weight: 29.5 kDa

Purity by SDS-PAGE: ≥95%

Purity by HPLC: N/A

Endotoxin Level: N/A

Biological Activity: 20-22 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one micromole of Meo-Suc-Ala-Pro-Val-pNA per minute at 25°C in 100 mM Tris-HCl, pH 7.5, with 500 mM NaCl.

Reconstitution: Deionized water

Storage Temp.: -20°C

Shipping: Gel pack

Background Information: Elastase, like trypsin and chymotrypsin, is a serine protease that also hydrolyses amides and esters. It is produced in the pancreas as an inactive zymogen, proelastase, and activated in the duodenum by trypsin. Elastase is distinctive in that it acts upon elastin.This enzyme degrades elastin, collagen, and proteoglycan and has been implicated in the development of pulmonary emphesema and rheumatoid arthritis.

Handling: Centrifuge the vial prior to opening.