Proteins and Peptides
EGFR Antibody (LS-B2914)
- 貨號 Cat. No.
- LS-B2914
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2914 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C84004. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | EGFR |
| Synonyms: | EGFR Antibody, egf receptor Antibody, egf-r Antibody, egfr Antibody, epidermal growth factor receptor Antibody, ERBB Antibody, erbb1 Antibody, HER1 Antibody, MENA Antibody, panitumumab Antibody, PIG61 Antibody, v-erb-b homolog Antibody |
| Family: | Protein Kinase |
| Subfamily: | EGF Receptor |
| Host: | EGFR antibody was produced in Sheep |
| Antigen Species: | EGFR antibody was raised against Human |
| Clonality: | Polyclonal |
| Antigen Type: | Synthetic peptide |
| Immunogen: | EGFR antibody was raised against a synthetic 20 amino acid peptide of human EGFr representing a portion of the internal domain proximal to a phosphorylation region |
| Antibody Specificity: | This EGFr antibody recognizes a region of the internal domain. Antibody binding blocks phosphorylation and does not interfere with EGF binding. |
| Antigen Modification: | Internal |
| Predicted Species Reactivity: | Chicken, Mouse, Rat |
| Purification: | Protein G Column |
| Presentation: | PBS buffer pH 7.4 |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2914 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2914 was determined to be 5 ug/ml. Western Blot: Dilution range 10 - 50 ug/mL dependent on receptor concentration. Immunoprecipitation (IP) - A431 epidermoid carcinoma 10 - 12 ug/mL. Protein kinase activity is measurable on the immunoprecipitated receptor. Immunocytochemistry (IC) Formalin fixed cells: 25 - 100 ug/mL dependent on receptor concentration. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Immunocytochemistry (ICC), Western blot (WB), Immunoprecipitation (IP) (10 - 12 µg/ml) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 1 mg/ml |
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