EGFR Antibody (LS-B2527)

貨號 Cat. No.: LS-B2527
規格 Size: 50 Âµl
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2527
Antibody Validation:	This antibody replaces catalog number LS-C6625. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Epidermal Growth Factor Receptor (EGFR)
Synonyms:	EGFR, egf receptor, egf-r, egfr, epidermal growth factor receptor, erbb1, panitumumab, v-erb-b homolog
Family:	Protein Kinase
Subfamily:	EGF Receptor
Host:	EGFR antibody was produced in Rabbit
Antigen Species:	EGFR antibody was raised against Human
Clonality:	Polyclonal
Isotype:	IgG
Antigen Type:	Synthetic peptide
Immunogen:	EGFR antibody was raised against synthetic peptide corresponding to residues surrounding Tyr1068 of human EGF receptor (KLH coupled).
Antibody Specificity:	Detects endogenous levels of total human EGF receptor protein at ~170kD. Does not cross-react with other proteins of the ErbB family. Species cross-reactivity: mouse and rat.
Predicted Species Reactivity:	Mouse, Rat
Purification:	Protein A Column
Presentation:	10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol. No preservative added.
Recommended Storage:	Long term: -20°C; Short term: +4°C
Antibody Usage Summary:	Immunohistochemistry: LS-B2527 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2527 was determined to be 1:50.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (1:50), Western blot (WB) (1:1000), Immunoprecipitation (IP) (1:50) (Optimal dilution to be determined by the researcher)
Size:	50 µl
Concentration:	0.13 mg/ml