EGF protein
- 貨號 Cat. No.
- EGF protein
- 規格 Size
- 500ug
- 品牌 Brand
- gene-rl
Description
| Product Name | EGF protein |
| Product type | Proteins and Peptides |
| Size | 500ug |
| Peptide | Recombinant full length protein, with a N-terminal hexahistidine tag. |
| Tested applications | SDS-PAGE, Western Blotting, Cell Test |
| Application notes | Recommended dilutions SDS-PAGE: Use at an assay dependent dilution Western Blotting: Use at an assay dependent dilution Optimal dilutions/concentrations should be determined by the end user. |
| Relevance | Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development. |
| Storage buffer | n/a |
| Purity | >95% by SDS-PAGE and PLC analysis. |
| Form | Lyophilised |
| Storage instructions | Aliquot and store at -20°C. Avoid freeze / thaw cycles. |
| Expression system | Yeast (Eukaryotic cell) |
| Sequence | Recombinant EGF is a 6.2 kDa protein containing 53 amino acid residues: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR |
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0ng/ml | 200ng/ml | 2ug/ml | 20ug/ml | ||||||
| Fig 1: EGF Treatment of 3T3 Cell Line 　　Different concentration of purified EGF was added into 3T3 cell culture medium. 　　Pictures were taken after 24 or 48 hours. 　　The purified growth factor increased the proliferation of 3T3 cell line. ã€€ã€€ç”Ÿé•·å› å在3T3的細胞ä¸ç”¨ä¸åŒæ¿ƒåº¦åŸ¹é¤Š,æ–¼24或48å°æ™‚æ‹ç…§è§€å¯Ÿ ã€€ã€€ç”Ÿé•·å› åå¯å¢žåŠ BeWo分化生長的能力 | |||||||||
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