eEF2 (A50) Antibody

貨號 Cat. No.
BS1099
規格 Size
100μg
品牌 Brand
Bioworld

Description

stock



Host: Rabbit


Reactivity: Human,Mouse,Rat


 



Product


1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.




Purification & Purity


The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).




Specificity


eEF2 (A50) antibody detects endogenous levels of eEF2 protein.




Applications (Recommended Dilutions)


WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200






Western Blot(WB)



Western blot (WB) analysis of eEF2 (A50) antibody in extracts from NIH/3T3 treated with serum 10% 30' cells.





Immunohistochemistry (IHC)



Immunohistochemistry (IHC) analyzes of eEF2 (A50) antibody in paraffin-embedded human breast carcinoma tissue.





Storage&Stability


Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.




Background


EEF2 is a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.


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