Proteins and Peptides
EDG3 Antibody (LS-B2155)
- 貨號 Cat. No.
- LS-B2155
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2155 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C22994. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Sphingosine-1-Phosphate Receptor 3 (S1PR3/EDG3) |
| Synonyms: | S1PR3, edg-3, edg3, endothelial differentiation, sphingolipid g-protein-coupled receptor, 3, S1P(3) receptor, S1P3, sphingolipid g-protein-coupled receptor 3 |
| Family: | GPCR |
| Subfamily: | Lysophospholipid/Lysosphingolipid |
| Host: | S1PR3 antibody was produced in Rabbit |
| Antigen Species: | S1PR3 antibody was raised against Human |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Synthetic peptide |
| Immunogen: | S1PR3 antibody was raised against synthetic peptide corresponding to aa12-25 [VRGNETLREHYQYV] of human EDG 3 protein. Species Sequence Homology: Mouse, rat: 79% (11/14). |
| Antibody Specificity: | Recognizes human EDG 3 (Endothelial Cell Differentiation Gene 63, Sphingosine-1-phosphate Receptor 3, S1PR3), ~47kD in human cerebral cortex. Species Cross-reactivity: Mouse and rat |
| Antigen Modification: | aa12-25 |
| Predicted Species Reactivity: | Mouse, Rat |
| Purification: | Immunoaffinity Chromatography |
| Presentation: | TBS, pH 7.4, 0.5 mg/ml BSA, 0.02% sodium azide, 50% glycerol. |
| Recommended Storage: | Long term: -20°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2155 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2155 was determined to be 10 ug/ml. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Immunocytochemistry (ICC), Western blot (WB) (3 µg/ml) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 0.6 mg/ml |
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