Proteins and Peptides
EBP1 Antibody (LS-B2210)
- 貨號 Cat. No.
- LS-B2210
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2210 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C22989. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Proliferation-associated 2G4, 38kD (PA2G4) |
| Synonyms: | PA2G4, cell cycle protein p38-2g4 homolog (hg4-1) |
| Family: | Exopeptidase |
| Subfamily: | Metallopeptidase M24C |
| Host: | PA2G4 antibody was produced in Rabbit |
| Antigen Species: | PA2G4 antibody was raised against Mouse |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Fusion protein |
| Immunogen: | PA2G4 antibody was raised against his-tagged fusion protein corresponding to residues 1-372 of mouse Ebp1 (ErbB-3 binding protein.) |
| Antibody Specificity: | Recognizes Ebp1, Mr ~48kD. An additional non-specific band of Mr ~120kD is detected on longer exposures or at high antibody concentrations. Species cross-reactivity: Human, mouse and monkey. |
| Antigen Modification: | aa1-372 |
| Predicted Species Reactivity: | Human, Monkey |
| Purification: | Protein A Column |
| Presentation: | PBS, pH 7.6, 0.07% sodium azide, 30% glycerol |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2210 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2210 was determined to be 5 ug/ml. Western Blot: 0.5-2 ug/ml Detects Ebp1 in HeLa Nuclear Extract. Immunoprecipitation: 2 ug immunoprecipitates Ebp1 from 500 ug of lysate. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (0.5 - 2 µg/ml), Immunoprecipitation (IP) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 1 mg/ml |
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