DYKDDDDK-Tag Protein ELISA Kit

Alternate Name :

Tag Line :

A competitive ELISA kit for the fast and sensitive quantitative detection of the expressed DYKDDDDK-tag proteins.

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection method- Absorbance (450 nm) • Species reactivity- Universal • Application- quantitative measurement of DYKDDDDK-Tag Protein in bacterial and mammalian cell lysates, downstream analyses of purification of DYKDDDDK-tag proteins.

Detection Method: Absorbance (650 nm)

Features & Benefits:

Detection Range: 256 – 10,000 ng/ml, In vitro, quantitative determination of DYKDDDDK-tag fusion proteins, Sensitivity: 100 ng/ml

Description:

DYKDDDDK tag has a small unique protein sequence that is commonly fused to N- or C-terminus of a protein for expression. Expression and detection of DYKDDDDK-tag proteins are frequently performed in many academic and industry laboratories because this tag is generally more hydrophilic than other common tags and therefore it is less likely to denature or inactivate proteins to which it is attached. In addition, DYKDDDDK-tag fusion proteins in E. coli or mammalian cell lysate can be easily purified by affinity chromatography using specific resins. Typically DYKDDDDK-tag proteins are detected by SDS-PAGE or western blots but these approaches are generally more laborious, time-consuming and less sensitive. BioVision’s DYKDDDDK-Tag Protein ELISA Kit is based on the competitive ELISA principle and it is an easy, fast and sensitive method to detect the expressed DYKDDDDK-tag proteins. This detection kit offers ready-to-use reagents, and can detect as low as 100 ng of DYKDDDDK-tag protein in approximately 1.5 hrs.