DNMT1 Antibody (LS-B2128)

貨號 Cat. No.: LS-B2128
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2128
Antibody Validation:	This antibody replaces catalog number LS-C520. It has been validated for use in the following assays: IHC-P.
Antibody Target:	DNA (cytosine-5-)-methyltransferase 1 (DNMT1)
Synonyms:	DNMT1
Family:	C5-methyltransferase
Subfamily:	Not assigned
Host:	DNMT1 antibody was produced in Mouse
Antigen Species:	DNMT1 antibody was raised against Human
Clonality:	Monoclonal
Isotype:	IgG1
Antibody Clone Name:	60B1220.1
Antigen Type:	Synthetic peptide - KLH conjugated
Immunogen:	DNMT1 antibody was raised against synthetic peptide
Antibody Specificity:	A synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1 (Genbank Accession No. NP_001370). It will cross react with mouse Dnmt1.
Antigen Modification:	aa637-650
Predicted Species Reactivity:	Mouse, Zebrafish
Purification:	Protein G Column
Presentation:	PBS, 0.05% BSA, 0.05% sodium azide.
Recommended Storage:	Long term: -20°C; Short term: +4°C
Antibody Usage Summary:	Immunohistochemistry: LS-B2128 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2128 was determined to be 5 ug/ml.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (2 - 4 µg/ml), Chromatin Immunoprecipitation (ChrIP), Immunoprecipitation (IP) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	0.5 mg/ml