DMAP1 Antibody

Antibody Target :DMAP1

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen Sequence: Synthetic peptide surrounding amino acid 438 of mouse DMAP1

Accession#: Q9NPF5

Gene ID: 55929

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.

Purification: Affinity purified

Species Reactivity: hu/ms/rt

Application: WB

Application & Usage: Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate (Cat.# 2401-100) and mouse small intestine tissue lysate (Cat.2601-100) can be used as positive controls. The antibody recognizes the DMAP1 of human, mouse and rat origins. Reactivity to other species has not been tested.

Storage Temp.: -20 °C

Shipping : gel pack

Shelf Life: 12 months

Handling: The antibody solution should be gently mixed before use.

Brief Background Descriptions: DNA methylation can help regulate transcriptional silencing via repressive transcription complexes, which include methyl-CpG-binding domain protein and Histone deacetylases (HDACs) DNMT1. This core enzyme for mammalian DNA methylation can also establish a repressive transcription complex consisting of DNMT1, HDAC2 and DMAP1. DMAP1 can interact directly with the transcriptional corepressor TSG101. The DNMT1-DMAP1 exists throughout the S-phase; HDAC2 joind DNMT1 and DMAP1 only during Late S phase. This provides a regulated means to deacetylate heterochromatin following replication