DFF45 Antibody
- 貨號 Cat. No.
- 3050-100
- 規格 Size
- 100 µg
Description
pfont size=2strongAntibody Target :/strongDFF45 /font/p
pstrongHost: Mouse /strong/p
pstrongAntibody Type: Monoclonal /strong/p
pstrongIsotype: Mouse IgG1 /strong/p
pstrongAntibody Form: Affinity Purified Antibody /strong/p
pstrongImmunogen Sequence: Recombinant human DFF45-GST fusion protein /strong/p
pstrongAccession#: NP_004392 /strong/p
pstrongGene ID: 1676 /strong/p
pstrongAppearance: Colorless liquid /strong/p
pstrongConcentration: 0.5 mg/ml /strong/p
pstrongFormulation: 100 micro;g (500 micro;g/ml) affinity purified antibody in PBS containing 50% glycerol, 0.5% BSA and 0.02% thimerosal. /strong/p
pstrongPurification: Affinity purified /strong/p
pstrongSpecies Reactivity: hu /strong/p
pstrongApplication: WB /strong/p
pstrongApplication amp; Usage: Western blot analysis (0.5-4 micro;g/ml). However, the optimal dilutions should be determined individually. Recognizes 45 and 40 KDa proteins, corresponding to the apparent molecular weight of DFF45/ICADL and DFF45/ICADs respectively. /strong/p
pstrongStorage Temp.: -20 deg;C /strong/p
pstrongShipping : gel pack /strong/p
pstrongShelf Life: 12 months /strong/p
pstrongHandling: The antibody solution should be gently mixed before use. /strong/p
pstrongBrief Background Descriptions: During apoptosis, various stimuli activate a protease cascade of caspases that eventually cause degradation of chromosomal DNA. DFF45 (DNA fragmentation factor 45) is a down-stream regulator of DNA degradation (DFF40). DFF40 is an apoptotic DNase with a high specific activity that exists in an inactive complex with DFF45 in living cells. Cleavage of DFF45, which is mediated by caspase-3, leads to DFF40 release where it then enters nucleus to function as a nuclease. DFF45 may inhibit the DNAse activity of DFF40 and its nuclear translocation. Activation of DFF40 causes DNA degradation, a hallmark of apoptosis./strongbr /
nbsp;/p
pstrongHost: Mouse /strong/p
pstrongAntibody Type: Monoclonal /strong/p
pstrongIsotype: Mouse IgG1 /strong/p
pstrongAntibody Form: Affinity Purified Antibody /strong/p
pstrongImmunogen Sequence: Recombinant human DFF45-GST fusion protein /strong/p
pstrongAccession#: NP_004392 /strong/p
pstrongGene ID: 1676 /strong/p
pstrongAppearance: Colorless liquid /strong/p
pstrongConcentration: 0.5 mg/ml /strong/p
pstrongFormulation: 100 micro;g (500 micro;g/ml) affinity purified antibody in PBS containing 50% glycerol, 0.5% BSA and 0.02% thimerosal. /strong/p
pstrongPurification: Affinity purified /strong/p
pstrongSpecies Reactivity: hu /strong/p
pstrongApplication: WB /strong/p
pstrongApplication amp; Usage: Western blot analysis (0.5-4 micro;g/ml). However, the optimal dilutions should be determined individually. Recognizes 45 and 40 KDa proteins, corresponding to the apparent molecular weight of DFF45/ICADL and DFF45/ICADs respectively. /strong/p
pstrongStorage Temp.: -20 deg;C /strong/p
pstrongShipping : gel pack /strong/p
pstrongShelf Life: 12 months /strong/p
pstrongHandling: The antibody solution should be gently mixed before use. /strong/p
pstrongBrief Background Descriptions: During apoptosis, various stimuli activate a protease cascade of caspases that eventually cause degradation of chromosomal DNA. DFF45 (DNA fragmentation factor 45) is a down-stream regulator of DNA degradation (DFF40). DFF40 is an apoptotic DNase with a high specific activity that exists in an inactive complex with DFF45 in living cells. Cleavage of DFF45, which is mediated by caspase-3, leads to DFF40 release where it then enters nucleus to function as a nuclease. DFF45 may inhibit the DNAse activity of DFF40 and its nuclear translocation. Activation of DFF40 causes DNA degradation, a hallmark of apoptosis./strongbr /
nbsp;/p