DARPP-32 Antibody
- 貨號 Cat. No.
- 3386-100
- 規格 Size
- 100 µg
Description
pstrongAntibody Target :/strongDARPP-32/p
pstrongHost: Rabbit /strong/p
pstrongAntibody Type: Polyclonal /strong/p
pstrongIsotype: Rabbit IgG /strong/p
pstrongAntibody Form: Affinity Purified Antibody /strong/p
pstrongImmunogen Sequence: Synthetic peptide derived from human DARPP-32 /strong/p
pstrongAccession#: AAB30129 /strong/p
pstrongGene ID: 545790 /strong/p
pstrongAppearance: Colorless liquid /strong/p
pstrongConcentration: 0.5 mg/ml /strong/p
pstrongFormulation: 100 micro;g (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal. /strong/p
pstrongPurification: Affinity purified /strong/p
pstrongSpecies Reactivity: hu/ms/rt /strong/p
pstrongApplication: WB /strong/p
pstrongApplication amp; Usage: Western blotting (0.5-4 micro;g/ml). However, the optimal concentrations should be determined individually. Rat brain cortex tissue lysate can be used as a positive control. The antibody recognizes 32 kDa DARPP-32 of human, mouse, and rat origins. /strong/p
pstrongStorage Temp.: -20 deg;C /strong/p
pstrongShipping : gel pack /strong/p
pstrongShelf Life: 12 months /strong/p
pstrongHandling: The antibody solution should be gently mixed before use. /strong/p
pstrongBrief Background Descriptions: DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein) is a cytosolic protein highly enriched spiny neurons of the neostriatum. DARPP-32 is a bifunctional signal transduction molecule that controls serine/threonine kinase and serine/threonine phosphatase activity depending on the phosphrylation state of the protein. Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at threonine 34 converts it into an inhibitor of protein phosphatase 1. DARPP-32 is converted into an inhibitor of PKA when phosphorylated at threonine 75 by cyclin-dependent kinase 5 (cdk5). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological and behavioral phenotype. /strong/p
pstrongHost: Rabbit /strong/p
pstrongAntibody Type: Polyclonal /strong/p
pstrongIsotype: Rabbit IgG /strong/p
pstrongAntibody Form: Affinity Purified Antibody /strong/p
pstrongImmunogen Sequence: Synthetic peptide derived from human DARPP-32 /strong/p
pstrongAccession#: AAB30129 /strong/p
pstrongGene ID: 545790 /strong/p
pstrongAppearance: Colorless liquid /strong/p
pstrongConcentration: 0.5 mg/ml /strong/p
pstrongFormulation: 100 micro;g (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal. /strong/p
pstrongPurification: Affinity purified /strong/p
pstrongSpecies Reactivity: hu/ms/rt /strong/p
pstrongApplication: WB /strong/p
pstrongApplication amp; Usage: Western blotting (0.5-4 micro;g/ml). However, the optimal concentrations should be determined individually. Rat brain cortex tissue lysate can be used as a positive control. The antibody recognizes 32 kDa DARPP-32 of human, mouse, and rat origins. /strong/p
pstrongStorage Temp.: -20 deg;C /strong/p
pstrongShipping : gel pack /strong/p
pstrongShelf Life: 12 months /strong/p
pstrongHandling: The antibody solution should be gently mixed before use. /strong/p
pstrongBrief Background Descriptions: DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein) is a cytosolic protein highly enriched spiny neurons of the neostriatum. DARPP-32 is a bifunctional signal transduction molecule that controls serine/threonine kinase and serine/threonine phosphatase activity depending on the phosphrylation state of the protein. Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at threonine 34 converts it into an inhibitor of protein phosphatase 1. DARPP-32 is converted into an inhibitor of PKA when phosphorylated at threonine 75 by cyclin-dependent kinase 5 (cdk5). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological and behavioral phenotype. /strong/p