D58A Ubiquitin
- 貨號 Cat. No.
- UM-D58A
- 規格 Size
- 1 mg
- 品牌 Brand
- Boston Biochem
Description
D58A Ubiquitin
human recombinant
Product Information
1 mg
Soluble and stable in aqueous buffers up to 10 mg/ml.
8.5 kDa
> 95% by SDS-PAGE
This is a lyophilized powder
Use & Storage
Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.
Store at -20°C after solubilization in desired buffer. Avoid multiple freeze/thaw cycles.
Catalog #: UM-D58A
D58A Ubiquitin
Product Source:
human recombinant
D58 is a residue that has been identified as being important for binding and recognition by proteins that contain ubiquitin binding domains (UBDs), and represents a new hydrophilic interaction surface on ubiquitin. This residue may be crucial for the interaction and recognition of poly-ubiquitin chains by the 26S proteasome and other enzymes involved in ubiquitination. Ub D58A can form an E1-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins.
Product Information
Quantity:
1 mg
Stock:
Soluble and stable in aqueous buffers up to 10 mg/ml.
MW:
8.5 kDa
Purity:
> 95% by SDS-PAGE
Supplied:
This is a lyophilized powder
Use & Storage
Use:
Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.
Storage:
Store at -20°C after solubilization in desired buffer. Avoid multiple freeze/thaw cycles.