D4-GDI Antibody
- 貨號 Cat. No.
- 3385-100
- 規格 Size
- 100 µg
Description
pstrongAntibody Target :/strongD4-GDI/p
pbHost: /bRabbit/p
pbAntibody Type: /bPolyclonal/p
pbIsotype: /bRabbit IgG/p
pbAntibody Form: /bAffinity Purified Antibody/p
pbImmunogen Sequence: /bSynthetic peptide near the N-terminus of human D4-GDI/p
pbAccession#: /bEAW96339/p
pbGene ID: /b397/p
pbAppearance: /bColorless liquid/p
pbConcentration: /b0.5 mg/ml/p
pbFormulation: /b100 micro;g (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal./p
pbPurification: /bAffinity purified/p
pbSpecies Reactivity: /bhu/p
pbApplication: /bWB/p
pbApplication amp; Usage: /bWestern blotting (0.5-4 micro;g/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 28 kDa D4-GDI in human samples. Jurkat cell lysate can be used as a positive control./p
pbStorage Temp.: /b-20 deg;C/p
pbShipping : /bgel pack/p
pbShelf Life: /b12 months/p
pbHandling: /bThe antibody solution should be gently mixed before use./p
pbBrief Background Descriptions: /bD4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeleytal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28kD mature form of D4-GDI to give a 23kDa and 5kDa size fragment. The 23kDa fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms./p
pbHost: /bRabbit/p
pbAntibody Type: /bPolyclonal/p
pbIsotype: /bRabbit IgG/p
pbAntibody Form: /bAffinity Purified Antibody/p
pbImmunogen Sequence: /bSynthetic peptide near the N-terminus of human D4-GDI/p
pbAccession#: /bEAW96339/p
pbGene ID: /b397/p
pbAppearance: /bColorless liquid/p
pbConcentration: /b0.5 mg/ml/p
pbFormulation: /b100 micro;g (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal./p
pbPurification: /bAffinity purified/p
pbSpecies Reactivity: /bhu/p
pbApplication: /bWB/p
pbApplication amp; Usage: /bWestern blotting (0.5-4 micro;g/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 28 kDa D4-GDI in human samples. Jurkat cell lysate can be used as a positive control./p
pbStorage Temp.: /b-20 deg;C/p
pbShipping : /bgel pack/p
pbShelf Life: /b12 months/p
pbHandling: /bThe antibody solution should be gently mixed before use./p
pbBrief Background Descriptions: /bD4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeleytal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28kD mature form of D4-GDI to give a 23kDa and 5kDa size fragment. The 23kDa fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms./p