Cytochrome c (H19) Antibody

貨號 Cat. No.

BS1089

規格 Size

100μg

品牌 Brand

Bioworld

Description

stock

Host: Rabbit

Reactivity: Human,Mouse,Rat

 

Product

1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Purification & Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity

Cytochrome c (H19) antibody detects endogenous levels of Cytochrome c protein.

Applications (Recommended Dilutions)

WB: 1:500~1:1000 IHC: 1:50~1:200

Western Blot(WB)

Western blot (WB) analysis of Cytochrome c (H19) antibody in extracts from COS7 cells.

Immunohistochemistry (IHC)

Immunohistochemistry (IHC) analyzes of Cytochrome c (H19) antibody in paraffin-embedded human colon carcinoma tissue.

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondri,a where it plays a critical role in the process of oxidative phosphorylation and production of cellular ATP. An increasing amount of interest has been directed toward the role which cytocrome C has been demonstrated to play in apoptotic processes. Following exposure to apoptotic stimuli, cytochrome C is rapidly released from the mitochondria into the cytosol, an event which may be required for the completion of apoptosis in some systems. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

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