Cytochrome c Antibody (LS-B2141)

貨號 Cat. No.: LS-B2141
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2141
Antibody Validation:	This antibody replaces catalog number LS-C514. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Cytochrome C, Somatic (CYCS)
Synonyms:	CYCS, cytochrome c
Host:	CYCS antibody was produced in Mouse
Antigen Species:	CYCS antibody was raised against Pigeon
Clonality:	Monoclonal
Isotype:	IgG2b
Antibody Clone Name:	7H8.2C12
Antigen Type:	Synthetic peptide - KLH conjugated
Immunogen:	CYCS antibody was raised against synthetic peptide
Antibody Specificity:	Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon CYT were used as the immunogen (Jemmerson et al. 1991). The antibody recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C.
Antigen Modification:	aa1-80
Predicted Species Reactivity:	Drosophila, Hamster, Horse, Human, Mouse, Rat
Purification:	Protein G Column
Presentation:	PBS, 0.2% gelatin, 0.05% sodium azide.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2141 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2141 was determined to be 5 ug/ml.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Immunocytochemistry (ICC), Western blot (WB) (1:500 - 1:1000) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	0.5 mg/ml