Cyclopiazonic Acid
- 貨號 Cat. No.
- C-750
- 規格 Size
- 10 x 1 mg 5mg 10mg 25mg
Description
Cyclopiazonic Acid
Apotent Inhibitor of Intracelluar Ca2+ - ATPase Pump
| Product#: | C-750 |
| Sizes: |
| 10 x 1 mg |
| 5 mg |
| 10 mg |
| 25 mg |
| M.W.: | 336.4 |
| Purity: | >98% |
| Bioassay: |
Cyclopiazonic acid (CPA #C-750) increases cytosolic Ca2+ in HL60 cell. |
 |
Intracellular Ca 2+ levels from Flou-3 AM loaded HL60 cells stimulated with increased concentration of CPA in precence of 4mM EGTA. |
| References: |
1. | Suzuki, M. et al. (1992 ) Br J Pharmacol. 107, 134. |
2. | Demaurex, N. et al. (1992)J. Biol. Chem. 267, 2318. |
3. | Zhang, P. et al.(1998) Nature 392, 835. |
4. | Xu, C. et al. (2002) J. Mol. Biol. 316, 201. |
5. | Moncoq, K. et al. (2007) J. Biol. Chem. 282, 9748. |
Cyclopiazonic acid (CPA) is a toxic fungal secondary metabolite. Chemically, it is an indole tetramic acid. CPA was originally isolated from Penicillium cyclopium and subsequently from other P. cyclopium, Penicillium griseofulvum, Penicillium camembertii, Aspergillus flavus and Aspergillus versicolor.
CPA is a highly potent, selective and reversible inhibitor of the sarcoplasmic reticulum (ER) Ca2+ - ATPase pump (SERCA). This Ca2+ pump is anintegral membrane protein of 994 amino acids that consists ofthree cytoplasmic domains containing the ATP binding site andten transmembrane helices containing the twocalcium binding sites.1,2
The interchange of Ca2+ between the cell cytosol and the ER lumen by SERCA is a central processinintracellularsignaling. Moreover, this process has a critical role at the muscle contraction-relaxationcycle, in which, the recovery phase relies on transport of calcium intothe ER by the action of the SERCA. Inhibition of this pump leads to decrease of Ca2+ uptake from the cytosol to the ER Lumen, thereby depletion of Ca2+ store operated and increase of cytosolic Ca2+. 3-4
CPA inhibits thecalcium pump by blocking the calcium access channel and immobilizinga subset of transmembrane helices. 5
Alomone Labs is pleased to offer Cyclopiazonic acid a natural toxin isolated from the Penicillium griseofulvum and purified to homogeneity.