Cyclin E1 (I119) Antibody

貨號 Cat. No.

BS1085

規格 Size

100μg

品牌 Brand

Bioworld

Description

stock

Host: Rabbit

Reactivity: Human,Mouse,Rat

 

Product

1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Purification & Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity

Cyclin E1 (I119) antibody detects endogenous levels of Cyclin E1 protein.

Applications (Recommended Dilutions)

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Western Blot(WB)

Western blot (WB) analysis of Cyclin E1 (I119) antibody in extracts from K562 cell.

Immunohistochemistry (IHC)

Immunohistochemistry (IHC) analyzes of Cyclin E1 (I119) antibody in paraffin-embedded human lung carcinoma tissue.

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background

Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. Cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. The cyclin E/E2F complex is detected primaril during the G1 phase of the cell cycle and decreases as cells enter S phase. E2F is known to be a critical transcription factor for expression of several S phase specific proteins.

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