Curcumenol

Description

Curcumenol, a sesquiterpene isolated from Curcuma zedoaria, is known to possess a variety of health and medicinal values which includes neuroprotection, anti-inflammatory, anti-tumor and hepatoprotective activities. It inhibits NF-κB activation by suppressing the nuclear translocation of the NF-κB p65 subunit and blocking IκBα phosphorylation and degradation.

Targets

In vitro

Curcumenol markedly decreases LPS-induced production of nitric oxide (NO), pro-inflammatory cytokines [(IL-6) and (TNF-α)] and pro-inflammatory proteins expression, iNOS and COX-2. Curcumenol has also been shown to exhibit hepatoprotective properties. Curcumenol can suppress the LPS-induced NF-κB activity by inhibiting the phosphorylation of Akt. Its treatment significantly inhibits LPS-induced phosphorylation of p38 MAPK but not JNK and ERK[1]. The activity of CYP3A4 is strongly inhibited by curcumenol with an IC50 value of 12.6 ± 1.3 μM. Kinetic analysis shows curcumenol competitively inhibits testosterone 6β-hydroxylation activity (CYP3A4) with Ki of 10.8 μM[2].

Cell Research:[1]

- Collapse

• 
  
  
  
  • Cell lines: BV-2 cell line
  
  
  • Concentrations: 2.5–20 μM
  
  
  • Incubation Time: 2 h
  
  
  • Method: BV-2 cells are seeded into a sterile flat bottom 96-well plate. The cells are then allowed to adhere overnight. The cells are pretreated with various concentrations of curcumenol for 2 h followed by LPS exposure (0.4 μg/mL) for 12 h to 24 h.
    (Only for Reference)