CUL1 / Cullin 1 Antibody (LS-B2448)

貨號 Cat. No.: LS-B2448
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2448
Antibody Validation:	This antibody replaces catalog number LS-C6247. It has been validated for use in the following assays: IHC-P.
Antibody Target:	CUL1 / Cullin 1
Synonyms:	CUL1 Antibody, cul1 Antibody, cullin 1 Antibody, MGC149834 Antibody, MGC149835 Antibody
Host:	CUL1 antibody was produced in Mouse
Antigen Species:	CUL1 antibody was raised against Human
Clonality:	Monoclonal
Isotype:	IgG1,k
Antigen Type:	Synthetic peptide
Immunogen:	CUL1 antibody was raised against a synthetic peptide corresponding to an 11 amino acid sequence at the C-terminus of the human CUL-1 protein.
Antibody Specificity:	Recognizes Cullin 1. Reacts specifically with the ~86kD CUL-1 protein and does not cross-react with the related CUL-2 protein. This antibody is confirmed to be reactive with human. Reactivity is confirmed from extracts of IMR90 (human fibroblasts) and Wi-38 cells.
Antigen Modification:	C-Terminus
Purification:	Protein A Column
Presentation:	PBS, pH 7.4, 0.1% sodium azide.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2448 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2448 was determined to be 10 ug/ml. Immunoprecipitation: 5-10 ug/ IP reaction.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Western blot (WB) (1 - 3 µg/ml), Immunoprecipitation (IP), ELISA (0.1 - 1 µg/ml) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	0.5 mg/ml