CUL-2 (I726) Antibody
- 貨號 Cat. No.
- BS1082
- 規格 Size
- 100μg
- 品牌 Brand
- Bioworld
Description
stock
Host: Rabbit
Reactivity: Human,Mouse
Product
1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
Purification & Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity
Cullin 2 (I726) antibody detects endogenous levels of Cullin 2 protein.
Applications (Recommended Dilutions)
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Western Blot(WB)
Western blot (WB) analysis of Cullin 2 (I726) antibody in extracts from LOVO cells.
Immunohistochemistry (IHC)
Immunohistochemistry (IHC) analyzes of Cullin 2 (I726) antibody in paraffin-embedded human lung carcinoma tissue.
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Background
Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of Cdc53 from yeast, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF(Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1 mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3 facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin- like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, or vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1.
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