CTNNB1 Antibody (LS-B2354)

貨號 Cat. No.: LS-B2354
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2354
Antibody Validation:	This antibody replaces catalog number LS-C21252. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Beta-catenin (CTNNB1)
Synonyms:	CTNNB1, beta cadherin associated protein, beta-catenin, cadherin-associated protein beta 1, ctnnb
Family:	Catenin
Subfamily:	not assigned-Catenin
Host:	CTNNB1 antibody was produced in Rabbit
Antigen Species:	CTNNB1 antibody was raised against Human
Clonality:	Polyclonal
Isotype:	IgG
Antigen Type:	Synthetic peptide
Immunogen:	CTNNB1 antibody was raised against a 15 amino acid synthetic peptide derived from the C-terminus of the human and mouse b-catenin protein.
Antibody Specificity:	Recognizes human b-catenin. Species cross-reactivity: chicken, mouse, rat and Xenopus.
Antigen Modification:	C-Terminus
Predicted Species Reactivity:	Chicken, Xenopus, Mouse, Rat
Purification:	Immunoaffinity Chromatography
Presentation:	PBS, pH 7.4, 0.1% sodium azide.
Recommended Storage:	Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
Antibody Usage Summary:	Immunohistochemistry: LS-B2354 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2354 was determined to be 2.5 ug/ml. Western Blot after IP: 1-2 ug/ml. Cross-reactivity with a ~100kD protein of unknown identity is sometimes observed. Immunoprecipitation: 5-10 ug. Specifically immunoprecipitate the ~92kD b-Catenin protein from native cell lysates. Positive control: HeLa and A431 lysates.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (2.5 µg/ml), Western blot (WB), Immunoprecipitation (IP), ELISA (0.1 - 1 µg/ml) (Optimal dilution to be determined by the researcher)
Size:	50 µg