CTGF (Mouse) ELISA Kit

Alternate Name : CCN2, HCS24, IGFBP8, Connective Tissue Growth Factor

Tag Line :

A Sandwich ELISA kit for the quantitative measurement of mouse CTGF in serum, plasma and other biological fluids,

Storage Conditions: 4ºC

Shelf life: 6 months

Summary :

Detection Method: Sandwich ELISA, Absorbance (450 nm)

Features & Benefits:

Sensitivity: 0.10 ng/mL,Precision: Coefficient of variation is < 10%,Specificity: No Significant cross-reactivity or interference between Mouse CTGF and analogues was observed.,Detection Range: 0.16-10 ng/mL

Description:

CTGF is a major connective tissue mitoattractant secreted by vascular endothelial cells. It promotes proliferation and differentiation of chondrocytes. It also mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells and enhances fibroblast growth factor-induced DNA synthesis. BioVision’s CTGF (Mouse) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CTGF. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Mouse CTGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of CTGF bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Mouse CTGF. The concentration of Mouse CTGF in the samples can be calculated by comparing the OD of the samples to the standard curve.