CSPG4 Antibody (LS-B2476)

貨號 Cat. No.: LS-B2476
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2476
Antibody Validation:	This antibody replaces catalog number LS-C22108. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Chondroitin Sulfate Proteoglycan 4 (melanoma-associated) (CSPG4)
Synonyms:	CSPG4, chondroitin sulfate proteoglycan 4, cspg4, HMW-MAA, MCSP, MCSPG, MEL-CSPG, melanoma-associated chondroitin sulfate proteoglycan, melanoma-specific chondroitin sulfate proteoglycan, MSK16, NG2
Host:	CSPG4 antibody was produced in Mouse
Antigen Species:	CSPG4 antibody was raised against Rat
Clonality:	Monoclonal
Isotype:	IgG1
Antigen Type:	Cells
Immunogen:	CSPG4 antibody was raised against cell line expressing a truncated form of NG2.
Antibody Specificity:	Recognizes rat NG2 Chondroitin Sulfate Proteoglycan. Recognizes native, recombinant and protein from cells which express NG2. Species sequence homology: human.
Purification:	Protein G Column
Presentation:	PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2476 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2476 was determined to be 5 ug/ml.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Immunohistochemistry - 4% paraformaldehyde (IHC-Pfa), Immunocytochemistry (ICC) (1:100 - 1:250), Western blot (WB), ELISA (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	0.5 mg/ml