CREB (D76D11) Rabbit mAb

Product Usage Information

For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

CREB (D76D11) Rabbit mAb detects endogenous levels of total CREB-1 protein. The antibody does not cross-react with other ATF/CREB family members.

Species Reactivity:

Human, Mouse, Rat, Hamster, Monkey, D. melanogaster

Source / Purification

Monoclonal antibody is produced by immunizing animals with a full length GST-CREB fusion protein.

Background

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).

1. Lonze, B.E. et al. (2002) Neuron 34, 371-85.


2. Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12.


3. Redmond, L. et al. (2002) Neuron 34, 999-1010.


4. Dash, P.K. et al. (1990) Nature 345, 718-21.


5. Yin, J.C. et al. (1994) Cell 79, 49-58.


6. Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8.


7. Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55.


8. Ribar, T.J. et al. (2000) J Neurosci 20, RC107.


9. Tan, Y. et al. (1996) EMBO J 15, 4629-42.