COPA Antibody (LS-B2566)

貨號 Cat. No.: LS-B2566
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2566
Antibody Validation:	This antibody replaces catalog number LS-C14583. It has been validated for use in the following assays: IHC-P.
Antibody Target:	COPA
Synonyms:	COPA Antibody, coatomer alpha subunit Antibody, FLJ26320 Antibody, HEP-COP Antibody
Host:	COPA antibody was produced in Rabbit
Antigen Species:	COPA antibody was raised against Human
Clonality:	Polyclonal
Isotype:	IgG
Antigen Type:	Synthetic peptide
Immunogen:	COPA antibody was raised against synthetic peptide: M(1)LTKFETKSARVKGLSFHP(19)C. Immunizing peptide corresponds to amino acid residues 1-19 from rat COP I alpha.
Antibody Specificity:	Detects rat coatomer-protein I alpha (COP I alpha). Species cross-reactivity: Human.
Antigen Modification:	aa1-19
Predicted Species Reactivity:	Rat
Purification:	Immunoaffinity Chromatography
Presentation:	PBS, 0.05% sodium azide.
Recommended Storage:	Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
Antibody Usage Summary:	Immunohistochemistry: LS-B2566 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2566 was determined to be 5 ug/ml. Western Blot: 1 ug/ml, detects an ~140kD protein representing COPI alpha from PC-12 cell extract. Also detects an unidentified protein at ~100kD.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Immunocytochemistry (ICC), Western blot (WB) (1 µg/ml) (Optimal dilution to be determined by the researcher)
Size:	50 µg