Collagen Type I (5D8) Antibody

h1 class=titleCollagen Type I (5D8) Antibody/h1
div id=node-472 class=node ntype-antibodiesMouse Monoclonal
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h4Catalog Number/h4
pstrong250274/strong/p
h4Unit/h4
pstrong0.2 mg /strong/p
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Format/h4
pEach vial contains 0.2 mg IgG in 0.2 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-A/G affinity chromatography./p
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h4Description/h4
pType I collagen is present in connective tissue and bone where it makes up about 90% of the organic matrix. Defects in one or both alleles of the type I collagen gene causes quot;brittle bone diseasequot; (osteogenesis imperfecta). Patients suffering from such defects have low levels of fully functional type I collagen resulting in fragile bones. This antibody is highly specific for native type I collagen and shows no evidence of cross-reactivity with type II, III, V and VI collagens in inhibition ELISA and immunoblotting, and no evidence of cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin). The antibody reacts with human, calf, sheep, pig, dog and goat type I collagens in ELISA and immunoblotting, but does not react with mouse, guinea pig, cat, rabbit, squirrel, horse, kangaroo or chicken type I collagen. The antibody has a Ka of approximately 2.5 x 106 M-1 for human type I collagen./p
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h4Product Citations/h4
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h4Alternate Names/h4
pCollagen alpha-1(I) chain; alpha-1 type I collagen; COL1A1/p
h4Accession No./h4
pa class=accession-link target=_blank href=http://www.expasy.org/uniprot/P02452P02452/abr /
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h4Clonality/h4
p5D8/p
h4Antigen/h4
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pPepsin-solubilized, salt-fractionated type I collagen from human placenta (1)/p
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h4Application Notes/h4
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pProduct Cat. No. 250274 can be used to detect collagens by ELISA. In immunoblotting Product Cat. No. 250274 detects human type I collagen only in its native triple helix form. Product Cat. No. 250274 can be used in immunohistochemistry with frozen unfixed sections of human and bovine skin. If tissue fixation is required, acetone or ethanol is recommended./p
pE: 1:16,000;/p
pWB: 1:1,000;/p
pIHC: 1:200/p
pstrongReacts with/strongnbsp; :H/p
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h4Storage/h4
pStore at 4deg;C. Product is guaranteed 6 months from the date of shipment./p
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