CMV AD169 Strain Quantitated Viral DNA

Virus: HCMV, strain AD169

Virus Preparation: Purified virus

Suspending Buffer: 10 mM Tris, 1 mM EDTA, pH 8.0

Cell Line for Propagation: Human foreskin fibroblasts (HFS)

PCR Analysis of DNA Control: PCR analysis was performed on purified CMV_AD169 DNA using the PE Biosystems GeneAmp^® Gold PCR Reagent Kit and primers specific to the glycoprotein 108 gene of CMV. The reaction produced a 292 bp fragment.

DNA Copy Number: 1-2 × 10⁴ copies/µL (varies by lot, please contact ABI for details)

DNA Quantitation: Viral DNA copy number is determined by real time PCR1,2,3,4 using the Roche LightCycler^®. DNA copy number may vary depending on the quantitation method used.

Application: ABI’s quantitated DNA controls are prepared from virus, bacteria, parasites, or mollicutes, and are intended for use as positive PCR quantitation standards for the organism in question.  Due to the nature of these products, ABI cannot guarantee their suitability as extraction controls.  Additionally, due to the extreme sensitivity of detection in PCR reaction, and since no method of purification can guarantee the complete absence of extraneous agents, DNA controls are not intended for use as negative controls for other organisms.