CMV AD169 Strain Purified Virus, β-PL Inactivated

Virus Preparation: Purified virus

Cell Line for Propagation: Human foreskin fibroblasts (HFS)

Suspending Buffer: 10mM Tris, 150mM NaCl, 1mM EDTA, pH 7.5 with gentamicin (25 µg/mL)

Final Fill Volume: Varies by lot

Protein Concentration: [varies by lot] mg/mL by Pierce BCA protein method using BSA standard. Alternative methods for determining protein concentration may give different values.

Virus Particle Count: Varies by lot

Sterility: Bacteria, yeast, fungi and Mycoplasma were not detected by our assay methods.

Validation of Inactivation: Beta-Propiolactone (β-PL) is a DNA alkylating agent that has been shown to be effective in the inactivation of viruses.  B-PL chemically modifies viral DNA without affecting the immunogenicity or protein structure.  After treatment with β-PL, infectious HCMV, strain AD169 was completely inactivated as validated by cell culture based assay (active HCMV, strain AD169 had a TCID₅₀ titer of 10^8.0 TCID₅₀/mL), but retained viral morphology (by TEM).