CMV AD169 Strain Purified Viral Lysate (1 mg/mL)

CMV, strain AD169 purified virus lysed with Triton X-100. Virus is completely inactivated and contains all viral proteins present in active preparation. Can be used for applications such as ELISA or Western blot requiring non-infectious viral proteins.

Virus: HCMV, strain AD169

Virus Preparation: Purified virus

Cell Line for Propagation: Human foreskin fibroblasts (HFS)

Suspending Buffer: 10 mM Tris, 150 mM NaCl, 1 mM EDTA, pH 7.5

Preparation of Lysate: HCMV purified virus adjusted to a protein concentration of approximately 1.0 mg/mL and Triton X-100 added to a final concentration of 0.5% in TNE buffer.

Protein Concentration: [varies by lot] mg/mL by Pierce BCA protein method using BSA standard. Alternative methods for determining protein concentration may give different values.

Functional Activity: Reactive in Western blot using HCMV pp28 and gB MAbs. Reactive in ELISA with HCMV MAbs to pp65, pp28, and gB.

Final Fill Volume: Varies by lot

Validation of Inactivation: This lot of viral lysate has been specifically tested for residual infectivity and no infectious virus was detected. The lysate was titered in HFS cells over a 12-14 day period with endpoint determination by cytopathic effect (CPE). This product has been treated using methods of inactivation that have been validated in vitro for complete inactivation of high-titered herpesviruses.