CLU Antibody (LS-B2218)

貨號 Cat. No.: LS-B2218
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2218
Antibody Validation:	This antibody replaces catalog number LS-C6185. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Clusterin (CLU)
Synonyms:	CLU, apoj, apolipoprotein j, clusterin, complement lysis inhibitor, complement-associated protein sp-40, sgp2, sulfated glycoprotein 2, testosterone-repressed prostate message 2, trpm2
Host:	CLU antibody was produced in Mouse
Antigen Species:	CLU antibody was raised against Human
Clonality:	Monoclonal
Isotype:	IgG1,k
Antigen Type:	Purified protein
Immunogen:	CLU antibody was raised against native clusterin purified from human serum.
Antibody Specificity:	Recognizes human clusterin, alpha at 40kD
Purification:	Protein G Column
Presentation:	0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%
Recommended Storage:	Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
Antibody Usage Summary:	Immunohistochemistry: LS-B2218 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2218 was determined to be 5 ug/ml. Western Blot: 0.5-2 ug/ml. Detects human clusterin a chain (40kD) in RIPA lysates from HeLa cells, human serum and MCF-7 cell lysates.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (0.5 - 2 µg/ml) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	0.5 mg/ml