Proteins and Peptides
CLDN5 Antibody (LS-B2331)
- 貨號 Cat. No.
- LS-B2331
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2331 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C6158. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Claudin 5 (CLDN5) |
| Synonyms: | CLDN5, claudin 5 |
| Family: | Claudin |
| Subfamily: | not assigned-Claudin |
| Host: | CLDN5 antibody was produced in Mouse |
| Antigen Species: | CLDN5 antibody was raised against Mouse |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Antigen Type: | Synthetic peptide |
| Immunogen: | CLDN5 antibody was raised against synthetic peptide derived from the mouse Claudin-5 protein. |
| Antibody Specificity: | Recognizes rat endogenous Claudin-5 protein at ~22-24kD. Species cross-reactivity: WB: mouse, IHC: human. |
| Predicted Species Reactivity: | Rat |
| Purification: | Protein A Column |
| Presentation: | PBS, pH 7.4, 0.1% sodium azide. |
| Recommended Storage: | Long term: Add glycerol (40-50%) -20°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2331 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2331 was determined to be 5-10 ug/ml. Positive control: Rat lung, kidney, and small intestine homogenates and mouse lung homogenates. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 - 10 µg/ml), Western blot (WB) (1 - 3 µg/ml), ELISA (0.1 - 1 µg/ml) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 0.5 mg/ml |
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